Possible Clues:

• Brass nedk or bile
• This bladder is attached to the liver and stores bile
• Cheek or nerve
• Bitterness when local girl has left
• Bold impudent behaviour; abnormal growth on tree
• Presumptuousness
• Everybody's come to the wrong conclusion - the cheek!
• Growth on tree; bile; resentment
• Sheer impudence from bladder?
• Irritate - presumption